ABSTRACT
Abstract Dimethoate is frequently used all over the world and it caused serious toxicity on target and non-target organisms. In this study, distilled water, ferulic acid, low dose dimetoate, high dose dimetoate, ferulic acid and low dose dimetoate, ferulic acid and high dose dimethoate were given to rats through gavage during the 4-week experiment. For this purpose, the levels of glutathione peroxidase, superoxide dismutase, glutathione-S-transferase, catalase, malondialdehyde and histopathological damages were investigated. After 28 days, no statistically important difference was determined in all investigated parameters in testicular tissues of rats who were administered control and ferulic acid. When the control and ferulic acid groups compare with the low and high dose dimetoate groups, there were statistically significantly changes antioxidant enzymes and malondialdehyde levels. In ferulic acid plus low dose dimethoate treated group and ferulic acid plus high dose dimethoate treatment we demonstrated that the protective role of ferulic acid on examining parameters but not completely. Based on light microscope studies, we can say that both dose dimethoate induced numerous histopathological changes. Less pathological alterations were observed when rats ferulic acid-plus-dimethoate. As a result, it is possible to say that ferulic acid has a partially healing role on the oxidative damage caused by dimethoate.
ABSTRACT
Objective: The study has been conducted to search out the threshold duration of treatment of ethyl acetate fraction of methanolic extract of leaves of Camellia sinensis (L.) Kuntze at the dose of 100 mg/kg body weight for the management of diabetes-induced testicular impairment in streptozotocin-induced diabetic rat in a duration dependent fashion. Methods: In this respect, the glycemic, androgenic, oxidative stress sensors, gene expression of testicular androgenic key enzymes along with apoptotic markers were evaluated in a duration dependent way (14, 28 and 56 d). Results: A significant correction was noted in the levels of glycated haemoglobin (HbA1C), testicular thiobarbituric acid reactive substances (TBARS), conjugated diene (CD), sperm viability, sperm mitochondrial status, serum testosterone, and genomic expression of testicular Δ5, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, Bax, Bcl-2 after treatment for different duration with the said fraction in diabetic groups in compare to respective vehicle-treated diabetic group without any toxicity induction in general. Thin layer chromatography (TLC) study of the fraction showed two spots with retention factors (Rf) of 0.78 and 0.51. Conclusion: The results showed that 28 d treatment was threshold duration of treatment for the correction of diabetes-induced testicular impairment.
ABSTRACT
Androgen deficiency is a physical disorder that not only affects adults but can also jeopardize children's health. Because there are many disadvantages to using traditional androgen replacement therapy, we have herein attempted to explore the use of human umbilical cord mesenchymal stem cells for the treatment of androgen deficiency. We transplanted CM-Dil-labeled human umbilical cord mesenchymal stem cells into the testes of an ethane dimethanesulfonate (EDS)-induced male rat hypogonadism model. Twenty-one days after transplantation, we found that blood testosterone levels in the therapy group were higher than that of the control group (P = 0.037), and using immunohistochemistry and flow cytometry, we observed that some of the CM-Dil-labeled cells expressed Leydig cell markers for cytochrome P450, family 11, subfamily A, polypeptide 1, and 3-β-hydroxysteroid dehydrogenase. We then recovered these cells and observed that they were still able to proliferate in vitro. The present study shows that mesenchymal stem cells from human umbilical cord may constitute a promising therapeutic modality for the treatment of male hypogonadism patients.
ABSTRACT
Aim: This study was designed to explore and exploit the phytotherapeutic and fertility effects of ethanolic leaf extract of Dracaena arborea in type-1 Alloxan-induced diabetic rats. The phytotherapeutic effects of Dracaena arborea on hematological parameters, appetite, spermiogram, histological architecture and histomorphometrics (stereology) of testicular and/or pancreatic tissues of treated and untreated rats were carried out. Place and Duration of Study: Department of Anatomy, College of Medicine, Lagos State University, Ikeja, Lagos, Nigeria, between October, 2012 and February, 2013. Methodology: 24 healthy normal male rats were recruited for this study. They were divided into four groups in a randomized trial; with group A consisting of non-diabetic healthy rats that received only the vehicle (0.5 mg/kg of 2% acacia solution); while group B, C & D was injected intraperitoneally with a single dose of Alloxan monohydrate (ALX) at 100 mg/kg prior to DAE treatment. Groups C and D were subsequently administered DAE orally 72 hours post administration of ALX, at daily doses of 100 mg/kg and 300 mg/kg respectively. Results: ALX (100 mg/kg) was found to induce type 1 diabetic conditions in the rats, demonstrated by the significant increase (P < 0.05) in the glucose levels, and a decline in appetite (water and food intakes). Conversely, administration of DAE at 100 and 300 mg/kg revealed significant dose and time- dependent increase (P < 0.05) in glucose tolerance and appetite (water and food intakes) in DAE treated groups compared to the untreated or ALX treated only group. Significant normalization (P < 0.05) of red blood cell count, packed cell volume and Hemoglobin levels were also observed in diabetic rats treated with the 100 mg/kg and 300 mg/kg DAE. In addition, testicular and pancreatic histopathological profiles of both DAE treated groups show evidences of appreciable normalization of ALX-induced pathology. Conclusion: Our findings indicates that DAE may offer great therapeutic benefit in the treatment of type-1 diabetes mellitus and normalizing testicular dysfunction or infertility in diabetic patients.
ABSTRACT
Twenty seven patients with micropenis including 15 patients who had micropenis accompanied by hypospadias were managed at Seoul National University Hospital during the recent seven years. Eleven patients had cryptorchidism, 9 of them were bilateral. Etiology was determined by hormonal assay including HCG stimulation test ; 9 as hypogonadotropic hypogonadism, 3 as primary testicular dysfunction, I as partial androgen insensitivity syndrome and 5 as idiopathic. Etiology was undertimined in 9 patients. One patients was found to be Prader-Willi syndrome and another one patient Kallmann`s syndrome. Four other patients had other anomalies , imperforate anus. cleft palate, hypertelorism and ptosis of eyelid, respectively. Nineteen patients had endocrine therapy using HCG, testosterone cream or Depotestosterone injection and all except one case respodeded satisfactorily in penile size increase.